Goat Anti-LRRK2 / PARK8 (internal) Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

dardarin|Parkinson disease (autosomal dominant) 8|ROCO2|PARK8|leucine-rich repeat kinase 2|LRRK2

NP_940980.2

EBP07172

Peptide with sequence C-NRKNTEGTQKQKE, from the internal region of the protein sequence according to NP_940980.2.

C-NRKNTEGTQKQKE

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Rat, Dog

120892

300160

Antibody detection limit dilution 1:32000.

Western Blot: Preliminary experiments gave an approx 200kDa band in Human Brain (Frontal Cortex) lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 286kDa according to NP_940980.2. The 200kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA