Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names Parkinson disease (autosomal dominant) 8|dardarin|leucine-rich repeat kinase 2|DKFZp434H2111|FLJ45829|ROCO2|PARK8|LRRK2 | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the vesicles of A431 cells and in the vesicles and nuclei of A549 cells. Recommended concentration: 10µg/ml. | |
Accession ID NP_940980.3 | |
Blocking Peptide EBP06550 | |
Immunogen Peptide with sequence CELAEKMRRTSV, from the internal region (near the C Terminus) of the protein sequence according to NP_940980.3. | |
Peptide Sequence CELAEKMRRTSV | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human | |
Reactive Species Human | |
Human Gene ID 120892 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:128000. | |
Western Blot Not yet tested - our routine western blotting protocol does not allow for the detection of proteins >250kDa (calculated MWt of approx. 286kDa according to NP_940980.3). However, customer testing has shown bands at >250kDa + 140kDa, which has been observed by other commercial sources of LRRK2 antibodies. | |
Application Type Pep-ELISA, IF |
Goat Anti-LRRK2 / PARK8 (near C Terminus) Antibody
$423.00
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Selected References [{"pmid": 19640926, "intro": "This antibody (previous batch) has been successfully used in IEM:", "title": "LRRK2 regulates autophagic activity and localizes to specific membrane microdomains in a novel human genomic reporter cellular model.", "author": "Alegre-Abarrategui J, Christian H, Lufino MM, Mutihac R, Venda LL, Ansorge O, Wade-Martins R.", "journal": "Hum Mol Genet. 2009 Nov 1;18(21):4022-34."}, {"pmid": 36219522, "intro": "This antibody has been successfully used in the following paper:", "title": "LRRK2 expression in normal and pathologic human gut and in rodent enteric neural cell lines.", "author": "Adrien De Guilhem De Lataillade, Martial Caillaud, Thibauld Oullier, Philippe Naveilhan, Carolina Pellegrini, Eduardo Tolosa, Michel Neunlist, Malvyne Rolli-Derkinderen, Ellen Gelpi, Pascal Derkinderen", "journal": "J Neurochem. 2023 Jan;164(2):193-209."}, {"pmid": 17971075, "intro": "This antibody (previous batch) has been successfully used in WB and IHC on Human:", "title": "LRRK2 is a component of granular alpha-synuclein pathology in the brainstem of Parkinson's disease.", "author": "Alegre-Abarrategui J, Ansorge O, Esiri M, Wade-Martins R.", "journal": "Neuropathol Appl Neurobiol. 2007 Oct 26."}, {"pmid": 21696411, "intro": "This antibody (previous batch) has been successfully used in ICC on Human:", "title": "LRRK2 expression in idiopathic and G2019S positive Parkinson's disease subjects: A morphological and quantitative study.", "author": "Sharma S, Bandopadhyay R, Lashley T, Renton AE, Kingsbury AE, Kumaran R, Kallis C, Vilariño-Güell C, O'Sullivan SS, Lees AJ, Revesz T, Wood NW, Holton JL.", "journal": "Neuropathol Appl Neurobiol. 2011 Jun 23."}] |