Goat Anti-Manic Fringe (mouse) Antibody

$255.00

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SKU: EB11554
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase|MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase|Mfng|manic fringe homolog|beta-1, 3-N-acetylglucosaminyltransferase manic fringe|AW546563|AL591946.1
Accession ID
NP_032621.1
Blocking Peptide
EBP11554
Immunogen
Peptide with sequence C-NRPIHASELQSKNR, from the internal region of the protein sequence according to NP_032621.1.
Peptide Sequence
C-NRPIHASELQSKNR
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Mouse, Rat
Mouse Gene ID
17305
Rat Gene ID
315119
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:8000.
Western Blot
Preliminary experiments gave an approx 100kDa band in Mouse Spleen lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 36.2kDa according to NP_032621.1. The 100kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Application Type
Pep-ELISA