Goat Anti-MARCH10 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB08235
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
MARCH10|membrane-associated ring finger (C3HC4) 10|FLJ35757|MARCH-X|RNF190|ring finger protein 190
Accession ID
NP_001094345.1; NP_689811.2
Blocking Peptide
EBP08235
Immunogen
Peptide with sequence C-SRNYPQPRTEE, from the internal region of the protein sequence according to NP_001094345.1; NP_689811.2.
Product Comments
This antibody is expected to recognise both reported isoforms (NP_001094345.1; NP_689811.2), which are identical.
Peptide Sequence
C-SRNYPQPRTEE
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Rat, Cow
Reactive Species
Human, Rat
Human Gene ID
162333
Rat Gene ID
303596
Product Grade
IHC Results
Paraffin embedded Human Testis. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:8000.
Western Blot
Approx. 90 kDa band observed in Rat Testis lysates (calculated MW of 90.5 kDa according to Human NP_689811.2 and of 87.9 kDa according to Mouse NP_766156.2). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunising peptide.
Application Type
Pep-ELISA, WB, IHC

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