Goat Anti-Mcp10 / Mcp9 Antibody

$255.00

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SKU: EB08817
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
Mcp10|Mast cell protease 10|Chymase 2, mast cell|Cma2
Accession ID
NP_001019885.1; NP_034912.3
Blocking Peptide
EBP08817
Immunogen
Peptide with sequence CKIFKHYKDSLQ, from the intenal region of the protein sequence according to NP_001019885.1; NP_034912.3.
Product Comments
Mcp 10 and Mcp 9 (GeneID 545055) are almost identical in their protein sequence. No cross-reactivity expected with the similar Mcp4.
Peptide Sequence
CKIFKHYKDSLQ
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Mouse
Mouse Gene ID
17232
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:2000.
Western Blot
Preliminary experiments gave an approx 49kDa band in Human Skeletal Muscle lysates after 0.02µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 26.9kDa according to NP_001019885.1 and NP_034912.3. The 49kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Application Type
Pep-ELISA