Goat Anti-mKIAA0319 (mouse) Antibody

$423.00

Category: Primary Antibodies
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SKU: EB07476
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
mKIAA0319|similar to KIAA0319 gene product|4930451E12Rik|KIAA0319
Accession ID
NP_001074520.1
Blocking Peptide
EBP07476
Immunogen
Peptide with sequence C-QGKIKQENKPTLH, from the internal region of the protein sequence according to NP_001074520.1.
Product Comments
The variants XP_341528.2 and XP_001070349.1 represent identical protein. This antibody is expected to cross-react with mouse KIAA0319 isoform 1 (NP_001074520.1), isoform 2 (XP_994023.1) and isoform 4 (XP_913491.1).
Peptide Sequence
C-QGKIKQENKPTLH
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Mouse, Rat
Reactive Species
Mouse
Mouse Gene ID
210108
Rat Gene ID
361244
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 150kDa band observed in Mouse Brain lysates (calculated MW of 118kDa according to Mouse NP_001074520.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Velayos-Baeza, Hum Mol Genet. 2008 Mar 15;17(6):859-71; PMID: 18063668). Recommended concentration: 0.5-1.5µg/ml. An additional band of unknown identity was also consistently observed at 25kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Application Type
Pep-ELISA, WB

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