Goat Anti-MKRN1 (aa105-118) Antibody

$464.00

Category: Primary Antibodies
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SKU: EB10898
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
RING finger protein 61| OTTHUMP00000212124| OTTHUMP00000212121| E3 ubiquitin-protein ligase makorin-1| RNF61| FLJ21334| makorin ring finger protein 1|MKRN1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of THP-1 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_038474.2; NP_001138597.1; NP_001278592.1
Blocking Peptide
EBP10898
Immunogen
Peptide with sequence C-RYEHSKPLKQEEAT, from the internet regoin of the protein sequence according to NP_038474.2; NP_001138597.1; NP_001278592.1.
Product Comments
This antibody is expected to recognize all reported isoforms (NP_038474.2; NP_001138597.1; NP_001278592.1).
Peptide Sequence
C-RYEHSKPLKQEEAT
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Cow
Reactive Species
Human
Human Gene ID
23608
Mouse Gene ID
54484
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Approx. 55kDa band observed in lysates of cell line THP-1 and in Human Testis lysates (calculated MW of 53.3kDa according to NP_038474.2). Recommended concentration: 0.1-0.5ug/ml. Primary incubation 1 hour at room temperature. Positive Control: A batch specific positive control lysate is available for this product. Please contact [email protected] for availability.
Application Type
Pep-ELISA, WB, IF, FC

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