Goat Anti-MOB2 (aa128-140) Antibody

$423.00

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SKU: EB10797
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
mps one binder kinase activator-like 2| mob2 homolog| HCCA2 protein| HCCA2|MOB2
Accession ID
NP_001165694.1; NP_443731.2
Blocking Peptide
EBP10797
Immunogen
Peptide with sequence DERGKKVKCTAPQ, from the internal region of the protein sequence according to NP_001165694.1; NP_443731.2.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_001165694.1; NP_443731.2) only.
Peptide Sequence
DERGKKVKCTAPQ
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow
Reactive Species
Human
Human Gene ID
81532
Mouse Gene ID
101513
Rat Gene ID
499288
Product Grade
IHC Results
In paraffin embedded Human Lung shows staining of smooth muscle cells, vasculature and apical secretory granules in the epithelium of the bronchiole. Recommended concentration: 5-10µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 30kDa band observed in lysates of cell line Jurkat (calculated MW of 30.2kDa according to NP_001165694.1). Recommended concentration: 0.02-0.06µg/ml. An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunizing peptide.
Application Type
Pep-ELISA, WB, IHC
Selected References
[{"pmid": 22566124, "intro": "This antibody has been successfully used in Western blot on Rat:", "title": "Mps one binder 2 gene upregulation in the stellation of astrocytes induced by cAMP-dependent pathway.", "author": "Fang KM, Liu YY, Lin CH, Fan SS, Tsai CH, Tzeng SF.", "journal": "J Cell Biochem. 2012 Sep;113(9):3019-28. doi: 10.1002/jcb.24180."}]