Goat Anti-MSH5 Antibody

$423.00

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SKU: EB08335
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
mutS homolog 5|NG23|MutSH5|G7N: MGC2939|DKFZp434C1615|XXbac-BCX40G17.5|mutS homolog 5 (E. coli)|MSH5
Accession ID
NP_079535.3 ; NP_751897.1; NP_002432.1
Blocking Peptide
EBP08335
Immunogen
Peptide with sequence GDLHCEIRDQET, from the internal region of the protein sequence according to NP_079535.3 ; NP_751897.1; NP_002432.1.
Product Comments
This antibody is expected to recognise three reported isoforms ( NP_079535.3 ; NP_751897.1; NP_002432.1); Reported variants NP_751898.1 and NP_002432.1 represent identical protein.
Peptide Sequence
GDLHCEIRDQET
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat
Reactive Species
Human
Human Gene ID
4439
Mouse Gene ID
17687
Rat Gene ID
294252
Product Grade
IHC Results
Paraffin embedded Human Testis and Thymus. Recommended concentration: 2.5-3.75µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Preliminary experiments gave an approx 38kDa band in Human Testis, Tonsil and Thymus lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.9kDa according to the smallest reported isoform NP_002432.1. The 38kDa band was successfully blocked by incubation with the immunizing peptide.
Application Type
Pep-ELISA, IHC