Goat Anti-MTHFD1 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

methylenetetrahydrofolate dehydrogenase 1|methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase|cytoplasmic C-1-tetrahydrofolate synthase|NADP-dependent cyclohydrolase/formyltetrahydrofolate synthetase|C1-THF synthase|5, 10-methylenetetrahydrofolate dehydrogenase, 5, 10-methylenetetrahydrofolate cyclohydrolase, 10-formyltetrahydrofolate synthetase|MTHFD|MTHFC|methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase|MTHFD1

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of MCF7 cells. Recommended concentration: 10µg/ml.

NP_005947.2; NP_001351766.1

EBP08489

Peptide with sequence RGDLNDCFIPCTPK, from the internal region of the protein sequence according to NP_005947.2; NP_001351766.1.

RGDLNDCFIPCTPK

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat

Human

4522

108156

64300

Paraffin embedded Human Liver. Recommended concentration: 3.75µg/ml.

Antibody detection limit dilution 1:32000.

Approx. 100kDa band observed in lysates of cell lines Jurkat and MOLT4 and approx.110kDa in lysates of cell line MCF7 (calculated MW of 102kDa according to NP_005947.2). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.

Pep-ELISA, WB, IHC, IF