Goat Anti-MTMR4 Antibody

$255.00

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SKU: EB05192
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
FYVE-DSP2|KIAA0647|myotubularin related protein 4|MTMR4
Usage Summary
Please note that the band we observe is different to the predicted MW of 131kDa. We do not have an explanation for this at present. Ishikawa et al., (DNA Res. 5: 169-176, 1998) observed MW of the in vitro transcribed/translated product of MTMR4 greater than 100 kD by SDS-PAGE. Zhao et al., (Exp. Cell Res. 265: 329-338, 2001) observed MW of MTMR4 to be approximately 130kDa. We would appreciate any feedback from people in the field - have any results been reported with other antibodies? Have any splice variants/modified forms been reported?
Accession ID
NP_004678
Blocking Peptide
EBP05192
Immunogen
Peptide with sequence C-MSQQLKKPIATASS, from the C Terminus of the protein sequence according to NP_004678.
Peptide Sequence
C-MSQQLKKPIATASS
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse
Reactive Species
Human
Human Gene ID
9110
Product Grade
Western Blot
Western blot: Approx 90-100kDa band seen in MOLT-4 cell lysates. Recommended for use at 1-3µg/ml.