Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names unc-13 homolog D|HPLH3|HLH3|FHL3|unc-13 homolog D (C. elegans)|Munc13-4|UNC13D | |
Accession ID NP_954712.1 | |
Blocking Peptide EBP07336 | |
Immunogen Peptide with sequence C-ETQKHKKDLHPLFD, from the internal region of the protein sequence according to NP_954712.1. | |
Peptide Sequence C-ETQKHKKDLHPLFD | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog, Pig, Cow | |
Reactive Species Human, Mouse | |
Human Gene ID 201294 | |
Mouse Gene ID 70450 | |
Rat Gene ID 192177 | |
Product Grade  | |
IHC Results In paraffin embedded Human Thymus shows cytoplasm staining in select areas. Recommended concentration: 2.5µg/ml.
In paraffin embedded Mouse Lung shows staining of the resperatory epithelium in wildtype but not in the knock-out. Data kindly provided by Micheal Tuvim, University of Texas MD Anderson Cancer Center, Houston, USA.
Recommended concentration: 1-2µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:128000. | |
Western Blot Approx. 105kDa band observed in Human T-lymphocyte and HeLa lysates (calculated MW of 123kDa according to NP_954712.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour. | |
Application Type Pep-ELISA, WB, IHC |
Goat Anti-Munc13-4 / UNC13D (Internal) Antibody
$423.00
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Selected References [{"pmid": 31032638, "intro": "This antibody has been successfully used in Western blot on Human:", "title": "Retroviral UNC13D Gene Transfer Restores Cytotoxic Activity of T Cells Derived from Familial Hemophagocytic Lymphohistiocytosis Type 3 Patients In Vitro.", "author": "Dettmer V, Bloom K, Gross M, Weissert K, Aichele P, Ehl S, Cathomen T", "journal": "Hum Gene Ther. 2019 Aug;30(8):975-984."}] |