Goat Anti-NANOG Antibody

$464.00

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SKU: EB06860
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
homeobox transcription factor Nanog-delta 48|homeobox transcription factor Nanog|HGNC:20857|Nanog homeobox|NANOG
Usage Summary
IF/ICC: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog (green).
This antibody was successfully used in IF on Human: PMID: 30878013, 21681858, 22613719, 21877920. 21285354,19890879, 19483674 and 18931654. This antibody was successfully used in ICC on Human: PMID: 31479876, 29034898 and 29034891. Enzyme immunoassay: This antibody does not work in PFA-fixed frozen embryos and ESC from Mouse.
Accession ID
NP_079141.2
Blocking Peptide
EBP06860
Immunogen
Peptide with sequence C-QNQRMKSKRWQKNN, from the internal region of the protein sequence according to NP_079141.2.
Peptide Sequence
C-QNQRMKSKRWQKNN
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Dog, Pig
Reactive Species
Human
Human Gene ID
79923
Mouse Gene ID
71950
Rat Gene ID
414065
Product Grade
IHC Results
This antibody was successfully used in: Vassena et al, Hum Mol Genet. 2012 Aug 1;21(15):3366-73. PMID: 22547223, Sancho-Martinez et al, Nat Commun. 2016 Feb 22;7:10743. PMID: 26899176, and Montserrat et al, Cell Transplant. 2012;21(5):815-25. PMID: 21944493.
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Approx 38kDa band observed in Human Ovary lysates (calculated MW of 34.6kDa according to NP_079141.2). Recommended concentration: 0.03-0.1µg/ml. Primary incubation was 1 hour.
Application Type
Pep-ELISA, WB, IF, IHC
Selected References
[{"pmid": 29034891, "intro": "This antibody has been successfully used in ICC on Human:", "title": "Generation of induced pluripotent stem cells (iPSC) from an atrial fibrillation patient carrying a KCNA5 p.D322H mutation", "author": "Cristina Mora, Marialaura Serzanti, Alessio Giacomelli, Valentina Turco, Eleonora Marchina, Valeria Bertini, Giovanna Piovani, Giulia Savio, Lena Refsgaard, Morten Salling Olesen, Venusia Cortellini, Patrizia Dell'Era", "journal": "Stem Cell Research 24 (2017) 29-32"}, {"pmid": 29034898, "intro": "This antibody has been successfully used in ICC on Human:", "title": "Generation of induced pluripotent stem cells (iPSC) from an atrial fibrillation patient carrying a PITX2 p.M200V mutation.", "author": "Mora C, Serzanti M, Giacomelli A, Beltramone S, Marchina E, Bertini V, Piovani G, Refsgaard L, Olesen MS, Cortellini V, Dell'Era P", "journal": "Stem Cell Res. 2017 Oct;24:8-11"}, {"pmid": 21877920, "intro": "This antibody has been successfully used in IF on Human:", "title": "Efficient Generation of A9 Midbrain Dopaminergic Neurons by Lentiviral Delivery of LMX1A in Human Embryonic Stem Cells and Induced Pluripotent Stem Cells", "author": "A. Sánchez-Danés, A. Consiglio, Y. Richaud, I. Rodríguez-Pizà, B. Dehay, M. Edel, J. Bové, M. Memo, M. Vila, A. Raya and J.C. Izpisua Belmonte", "journal": "Hum Gene Ther. 2012 Jan; 23(1): 56–69."}, {"pmid": 31479876, "intro": "This antibody has been successfully used in ICC on Human:", "title": "Generation of the induced human pluripotent stem cell lines CSSi009-A from a patient with GNB5 pathogenic variant, and CSSi010-A from a CRISPR/Cas9 engineered GNB5 knock-out human cell line.", "author": "N. Malerba, P. Benzoni, G.M. Squeo, et al", "journal": "Stem Cell Research (2019), https://doi.org/10.1016/j.scr.2019.101547."}, {"pmid": 30878013, "intro": "This antibody has been successfully used in IF on Human:", "title": "Generation of two transgene-free human iPSC lines from CD133+ cord blood cells (2019)", "author": "Estibaliz Arellano-Viera, Lorea Zabaleta, Julio Castaño, Garikoitz Azkona, Xonia Carvajal-Vergara, Alessandra Giorgetti", "journal": "https://doi.org/10.1016/j.scr.2019.101410"}, {"pmid": 26899176, "intro": "This antibody has been successfully used in IHC on Human:", "title": "Establishment of human iPSC-based models for the study and targeting of glioma initiating cells", "author": "Sancho-Martinez I, Nivet E, Xia Y, Hishida T, Aguirre A, Ocampo A, Ma L1, Morey R, Krause MN, Zembrzycki A, Ansorge O5 Vazquez-Ferrer E ,Dubova I1. Reddy P, Lam D, Hishida Y, Wu MZ, Esteban CR, O'Leary D, Wahl GM, Verma IM, Laurent LC, Izpisua Belmonte JC", "journal": "Nat Commun. 2016 Feb 22;7:10743"}, {"pmid": 21681858, "intro": "This antibody has been successfully used in IF on Human:", "title": "Complete meiosis from human induced pluripotent stem cells.", "author": "Eguizabal C, Montserrat N, Vassena R, Barragan M, Garreta E, Garcia-Quevedo L, Vidal F, Giorgetti A, Veiga A, Izpisua Belmonte JC.", "journal": "Stem Cells. 2011 Aug;29(8):1186-95."}, {"pmid": 24317394, "intro": "This antibody has been successfully used on Human:", "title": "Patient-specific naturally gene-reverted induced pluripotent stem cells in recessive dystrophic epidermolysis bullosa.", "author": "Tolar J, McGrath JA, Xia L, Riddle MJ, Lees CJ, Eide C, Keene DR, Liu L, Osborn MJ, Lund TC, Blazar BR, Wagner JE.", "journal": "J Invest Dermatol. 2014 May;134(5):1246-54."}, {"pmid": 21944493, "intro": "This antibody has been successfully used in IHC on Pig:", "title": "Generation of feeder-free pig induced pluripotent stem cells without Pou5f1.", "author": "Montserrat N, de Oñate L, Garreta E, González F, Adamo A, Eguizábal C, Häfner S, Vassena R, Izpisua Belmonte JC.", "journal": "Cell Transplant. 2012;21(5):815-25."}, {"pmid": 22547223, "intro": "This antibody has been successfully used in IHC on Human:", "title": "Accumulation of instability in serial differentiation and reprogramming of parthenogenetic human cells.", "author": "Vassena R, Montserrat N, Carrasco Canal B, Aran B, de Oñate L, Veiga A, Izpisua Belmonte JC.", "journal": "Hum Mol Genet. 2012 Aug 1;21(15):3366-73."}, {"pmid": 22613719, "intro": "This antibody has been successfully used in IF on Human:", "title": "Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, oct4 and sox2.", "author": "Montserrat N, Ramírez-Bajo MJ, Xia Y, Sancho-Martinez I, Moya-Rull D, Miquel-Serra L, Yang S, Nivet E, Cortina C, González F, Izpisua Belmonte JC, Campistol JM.", "journal": "J Biol Chem. 2012 Jul 13;287(29):24131-8."}, {"pmid": 21088946, "intro": "This antibody has been successfully used in IF on Pig:", "title": "Generation of pig iPS cells: a model for cell therapy.", "author": "Montserrat N, Bahima EG, Batlle L, Häfner S, Rodrigues AM, González F, Belmonte JC.", "journal": "J Cardiovasc Transl Res. 2011 Apr;4(2):121-30."}, {"pmid": 21285354, "intro": "This antibody has been successfully used in ICC/IF on Human:", "title": "Simple generation of human induced pluripotent stem cells using poly-beta-amino esters as the non-viral gene delivery system.", "author": "Montserrat N, Garreta E, González F, Gutiérrez J, Eguizábal C, Ramos V, Borrós S, Izpisua Belmonte JC.", "journal": "J Biol Chem. 2011 Apr 8;286(14):12417-28."}, {"pmid": 19890879, "intro": "This antibody has been successfully used in ICC/IF on Human:", "title": "Reprogramming of human fibroblasts to induced pluripotent stem cells under xeno-free conditions.", "author": "Rodríguez-Pizà I, Richaud-Patin Y, Vassena R, González F, Barrero MJ, Veiga A, Raya A, Belmonte JC.", "journal": "Stem Cells. 2010 Jan;28(1):36-44."}, {"pmid": 19483674, "intro": "This antibody has been successfully used in IF on Human:", "title": "Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells.", "author": "Raya A et.al.", "journal": "Nature. 2009 Jul 2;460(7251):53-9."}, {"pmid": 18931654, "intro": "This antibody has been successfully used in ICC/IF on Human:", "title": "Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes.", "author": "Aasen T, Raya A, Barrero MJ, Garreta E, Consiglio A, Gonzalez F, Vassena R, Bili? J, Pekarik V, Tiscornia G, Edel M, Boué S, Izpisúa Belmonte JC.", "journal": "Nat Biotechnol. 2008 Nov;26(11):1276-84."}]