Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names homeobox transcription factor Nanog-delta 48|homeobox transcription factor Nanog|HGNC:20857|Nanog homeobox|NANOG | |
Usage Summary IF/ICC: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog (green). This antibody was successfully used in IF on Human: PMID: 30878013, 21681858, 22613719, 21877920. 21285354,19890879, 19483674 and 18931654. This antibody was successfully used in ICC on Human: PMID: 31479876, 29034898 and 29034891. Enzyme immunoassay: This antibody does not work in PFA-fixed frozen embryos and ESC from Mouse. | |
Accession ID NP_079141.2 | |
Blocking Peptide EBP06860 | |
Immunogen Peptide with sequence C-QNQRMKSKRWQKNN, from the internal region of the protein sequence according to NP_079141.2. | |
Peptide Sequence C-QNQRMKSKRWQKNN | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Dog, Pig | |
Reactive Species Human | |
Human Gene ID 79923 | |
Mouse Gene ID 71950 | |
Rat Gene ID 414065 | |
Product Grade ![]() | |
IHC Results This antibody was successfully used in: Vassena et al, Hum Mol Genet. 2012 Aug 1;21(15):3366-73. PMID: 22547223, Sancho-Martinez et al, Nat Commun. 2016 Feb 22;7:10743. PMID: 26899176, and Montserrat et al, Cell Transplant. 2012;21(5):815-25. PMID: 21944493. | |
ELISA Detection Limit Antibody detection limit dilution 1:64000. | |
Western Blot Approx 38kDa band observed in Human Ovary lysates (calculated MW of 34.6kDa according to NP_079141.2). Recommended concentration: 0.03-0.1µg/ml. Primary incubation was 1 hour. | |
Application Type Pep-ELISA, WB, IF, IHC |
Goat Anti-NANOG Antibody
$464.00
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Selected References [{"pmid": 29034891, "intro": "This antibody has been successfully used in ICC on Human:", "title": "Generation of induced pluripotent stem cells (iPSC) from an atrial fibrillation patient carrying a KCNA5 p.D322H mutation", "author": "Cristina Mora, Marialaura Serzanti, Alessio Giacomelli, Valentina Turco, Eleonora Marchina, Valeria Bertini, Giovanna Piovani, Giulia Savio, Lena Refsgaard, Morten Salling Olesen, Venusia Cortellini, Patrizia Dell'Era", "journal": "Stem Cell Research 24 (2017) 29-32"}, {"pmid": 29034898, "intro": "This antibody has been successfully used in ICC on Human:", "title": "Generation of induced pluripotent stem cells (iPSC) from an atrial fibrillation patient carrying a PITX2 p.M200V mutation.", "author": "Mora C, Serzanti M, Giacomelli A, Beltramone S, Marchina E, Bertini V, Piovani G, Refsgaard L, Olesen MS, Cortellini V, Dell'Era P", "journal": "Stem Cell Res. 2017 Oct;24:8-11"}, {"pmid": 21877920, "intro": "This antibody has been successfully used in IF on Human:", "title": "Efficient Generation of A9 Midbrain Dopaminergic Neurons by Lentiviral Delivery of LMX1A in Human Embryonic Stem Cells and Induced Pluripotent Stem Cells", "author": "A. Sánchez-Danés, A. Consiglio, Y. Richaud, I. Rodríguez-Pizà, B. Dehay, M. Edel, J. Bové, M. Memo, M. Vila, A. Raya and J.C. Izpisua Belmonte", "journal": "Hum Gene Ther. 2012 Jan; 23(1): 56–69."}, {"pmid": 31479876, "intro": "This antibody has been successfully used in ICC on Human:", "title": "Generation of the induced human pluripotent stem cell lines CSSi009-A from a patient with GNB5 pathogenic variant, and CSSi010-A from a CRISPR/Cas9 engineered GNB5 knock-out human cell line.", "author": "N. Malerba, P. Benzoni, G.M. Squeo, et al", "journal": "Stem Cell Research (2019), https://doi.org/10.1016/j.scr.2019.101547."}, {"pmid": 30878013, "intro": "This antibody has been successfully used in IF on Human:", "title": "Generation of two transgene-free human iPSC lines from CD133+ cord blood cells (2019)", "author": "Estibaliz Arellano-Viera, Lorea Zabaleta, Julio Castaño, Garikoitz Azkona, Xonia Carvajal-Vergara, Alessandra Giorgetti", "journal": "https://doi.org/10.1016/j.scr.2019.101410"}, {"pmid": 26899176, "intro": "This antibody has been successfully used in IHC on Human:", "title": "Establishment of human iPSC-based models for the study and targeting of glioma initiating cells", "author": "Sancho-Martinez I, Nivet E, Xia Y, Hishida T, Aguirre A, Ocampo A, Ma L1, Morey R, Krause MN, Zembrzycki A, Ansorge O5 Vazquez-Ferrer E ,Dubova I1. Reddy P, Lam D, Hishida Y, Wu MZ, Esteban CR, O'Leary D, Wahl GM, Verma IM, Laurent LC, Izpisua Belmonte JC", "journal": "Nat Commun. 2016 Feb 22;7:10743"}, {"pmid": 21681858, "intro": "This antibody has been successfully used in IF on Human:", "title": "Complete meiosis from human induced pluripotent stem cells.", "author": "Eguizabal C, Montserrat N, Vassena R, Barragan M, Garreta E, Garcia-Quevedo L, Vidal F, Giorgetti A, Veiga A, Izpisua Belmonte JC.", "journal": "Stem Cells. 2011 Aug;29(8):1186-95."}, {"pmid": 24317394, "intro": "This antibody has been successfully used on Human:", "title": "Patient-specific naturally gene-reverted induced pluripotent stem cells in recessive dystrophic epidermolysis bullosa.", "author": "Tolar J, McGrath JA, Xia L, Riddle MJ, Lees CJ, Eide C, Keene DR, Liu L, Osborn MJ, Lund TC, Blazar BR, Wagner JE.", "journal": "J Invest Dermatol. 2014 May;134(5):1246-54."}, {"pmid": 21944493, "intro": "This antibody has been successfully used in IHC on Pig:", "title": "Generation of feeder-free pig induced pluripotent stem cells without Pou5f1.", "author": "Montserrat N, de Oñate L, Garreta E, González F, Adamo A, Eguizábal C, Häfner S, Vassena R, Izpisua Belmonte JC.", "journal": "Cell Transplant. 2012;21(5):815-25."}, {"pmid": 22547223, "intro": "This antibody has been successfully used in IHC on Human:", "title": "Accumulation of instability in serial differentiation and reprogramming of parthenogenetic human cells.", "author": "Vassena R, Montserrat N, Carrasco Canal B, Aran B, de Oñate L, Veiga A, Izpisua Belmonte JC.", "journal": "Hum Mol Genet. 2012 Aug 1;21(15):3366-73."}, {"pmid": 22613719, "intro": "This antibody has been successfully used in IF on Human:", "title": "Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, oct4 and sox2.", "author": "Montserrat N, Ramírez-Bajo MJ, Xia Y, Sancho-Martinez I, Moya-Rull D, Miquel-Serra L, Yang S, Nivet E, Cortina C, González F, Izpisua Belmonte JC, Campistol JM.", "journal": "J Biol Chem. 2012 Jul 13;287(29):24131-8."}, {"pmid": 21088946, "intro": "This antibody has been successfully used in IF on Pig:", "title": "Generation of pig iPS cells: a model for cell therapy.", "author": "Montserrat N, Bahima EG, Batlle L, Häfner S, Rodrigues AM, González F, Belmonte JC.", "journal": "J Cardiovasc Transl Res. 2011 Apr;4(2):121-30."}, {"pmid": 21285354, "intro": "This antibody has been successfully used in ICC/IF on Human:", "title": "Simple generation of human induced pluripotent stem cells using poly-beta-amino esters as the non-viral gene delivery system.", "author": "Montserrat N, Garreta E, González F, Gutiérrez J, Eguizábal C, Ramos V, Borrós S, Izpisua Belmonte JC.", "journal": "J Biol Chem. 2011 Apr 8;286(14):12417-28."}, {"pmid": 19890879, "intro": "This antibody has been successfully used in ICC/IF on Human:", "title": "Reprogramming of human fibroblasts to induced pluripotent stem cells under xeno-free conditions.", "author": "Rodríguez-Pizà I, Richaud-Patin Y, Vassena R, González F, Barrero MJ, Veiga A, Raya A, Belmonte JC.", "journal": "Stem Cells. 2010 Jan;28(1):36-44."}, {"pmid": 19483674, "intro": "This antibody has been successfully used in IF on Human:", "title": "Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells.", "author": "Raya A et.al.", "journal": "Nature. 2009 Jul 2;460(7251):53-9."}, {"pmid": 18931654, "intro": "This antibody has been successfully used in ICC/IF on Human:", "title": "Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes.", "author": "Aasen T, Raya A, Barrero MJ, Garreta E, Consiglio A, Gonzalez F, Vassena R, Bili? J, Pekarik V, Tiscornia G, Edel M, Boué S, Izpisúa Belmonte JC.", "journal": "Nat Biotechnol. 2008 Nov;26(11):1276-84."}] |


