Goat Anti-NCF1 / p47phox Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

NADPH oxidase organizer 2|neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1)|Neutrophil cytosolic factor-1 (47kD)|neutrophil cytosolic factor 1|SH3PXD1A|NOXO2|NCF1A|FLJ79451|p47phox|NCF1

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

NP_000256.4

EBP05054

Peptide with sequence C-SESTKRKLASAV, from the C Terminus of the protein sequence according to NP_000256.4.

C-SESTKRKLASAV

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Pig, Cow

Human, Mouse, Pig

653361

17969

114553

Paraffin embedded Human Colon, Neutrophils. Recommended concentration: 5µg/ml.

Antibody detection limit dilution 1:20000.

Approx 48kDa band observed in lysates of cell lines Daudi, U251 and U937 and in Mouse Thymus, and approx, 50kDa in Pig Spleen lysates (calculated MW of 44.7kDa according to Human NP_000256.4, 46.0kDa according to Mouse NP_001272966.1 and 45.3kDa according to Pig NP_001106691.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.01-1µg/ml. Primary incubation 1 hour at room temperature. Negative Control: A431 cell lysate. Preliminary testing was unsuccessful on Rat Spleen and Thymus for this particular batch.

Pep-ELISA, WB, IHC, IF, FC