Goat Anti-NDUFS1 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

NDUFS1|PRO1304|OTTHUMP00000206345|NADH-coenzyme Q reductase|NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase)|NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa|MGC26839|complex I, mitochondrial respirato|CI-75Kd

Immunofluorescence: Strong expression of the protein seen in U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

NP_004997.4; NP_001186910.1; NP_001186911.1; NP_001186912.1; NP_001186913.1

EBP10188

Peptide with sequence C-TEKSATYVNTEGR, from the internal region of the protein sequence according to NP_004997.4; NP_001186910.1; NP_001186911.1; NP_001186912.1; NP_001186913.1.

This antibody is expected to recognise isoforms NP_004997.4; NP_001186910.1; NP_001186911.1; NP_001186912.1; NP_001186913.1

C-TEKSATYVNTEGR

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Cow

Human, Mouse, Rat

4719

227197

301458

Paraffin embedded Human Heart. Recommended concentration: 5µg/ml.

Antibody detection limit dilution 1:64000.

Approx. 75kDa band observed in lysates of cell lines HEK293 and NIH3T3 and in Rat Heart lysates, and approx. 80kDa in Human and Mouse Heart and in Human Skeletal Muscle lysates (calculated MW of 79.5kDa according to Human NP_004997.4 and Rat NP_001005550.1, 75.4kDa according to Human NP_001186910.1, and 79.8kDa according to Mouse NP_001153510.1). Recommended concentration: 0.1-0.5µg/ml. Primary incubation 1 hour at room temperature.

Pep-ELISA, WB, IHC, IF, FC