Goat Anti-NEBL / nebulette Antibody

$255.00

Category: Primary Antibodies
SKU Unit Size Price

Select a unit size:

SKU: EB09459
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
actin-binding Z-disk protein|OTTHUMP00000019268|NEBL, actin-binding Z-disc protein|LIM-nebulette|bA56H7.1|MGC119747|MGC119746|LNEBL|FLJ53769|nebulette|NEBL
Accession ID
NP_998734.1; NP_001166955.1
Blocking Peptide
EBP09459
Immunogen
Peptide with sequence C-ELQRLKRTQE, from the internal region of the protein sequence according to NP_998734.1; NP_001166955.1.
Product Comments
This antibody is expected to recognize isoform 2 and isoform 3 (NP_998734.1; NP_001166955.1) only, but does not recognize isoform 1 (NP_006384.1).
Peptide Sequence
C-ELQRLKRTQE
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Dog
Human Gene ID
10529
Mouse Gene ID
74103
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:4000.
Western Blot
Preliminary experiments gave an approx 30kDa band in Human Brain (Frontal Cortex) lysates, but not in Human, Mouse and Rat Heart lysates after 1µg/ml antibody staining. This protein has a calculated MW of 31.2kDa according to NP_998734.1. The 30kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Application Type
Pep-ELISA

Related products