Goat Anti-Neuro-d4 / DPF1 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB05827
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
neuro-d4 homolog|OTTHUMP00000211615|BAF45b|MGC150429|MGC150428|D4, zinc and double PHD fingers, family 1|Neuro-d4 (rat) homolog|D4, zinc and double PHD fingers family 1|neuro-d4|NEUD4|DPF1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei and weaker staining in the cytoplasm of MCF7 and U2OS cells. Recommended concentration: 10µg/ml.
Accession ID
NP_001128627.1; NP_004638.2; NP_001128628.1
Blocking Peptide
EBP05827
Immunogen
Peptide with sequence C-HLKEKASAYITLT, from the C Terminus of the protein sequence according to NP_001128627.1; NP_004638.2; NP_001128628.1.
Product Comments
This antibody is expected to recognize all three reported isoforms (NP_001128627.1; NP_004638.2; NP_001128628.1).
Peptide Sequence
C-HLKEKASAYITLT
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Cow
Reactive Species
Human
Human Gene ID
8193
Mouse Gene ID
29861
Rat Gene ID
50545
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:2000.
Western Blot
Preliminary experiments showed a band at approx 38-40kDa in Human Cerebellum and Frontal Cortex lysates after 0.3-1µg/ml antibody staining (calculated MW of 37.9kDa according to NP_001128628.1. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, IF

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