Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names neurogenic locus notch homolog protein 4| Notch homolog 4| NOTCH3| MGC74442| INT3| FLJ16302| DAQB-143M3.4|NOTCH4 | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the nuclei and membranes of HeLa cells, and in the membranes of NIH3T3 cells. Recommended concentration: 10µg/ml. | |
Accession ID NP_004548.3 | |
Blocking Peptide EBP10786 | |
Immunogen Peptide with sequence DHFHNGHCEKGCNN, from the internal region of the protein sequence according to NP_004548.3. | |
Product Comments The immunizong peptide represents part of the extracellular domain. | |
Peptide Sequence DHFHNGHCEKGCNN | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog, Pig, Cow | |
Reactive Species Human, Mouse | |
Human Gene ID 4855 | |
Mouse Gene ID 18132 | |
Rat Gene ID 406162 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:8000. | |
Western Blot Preliminary experiments gave an approx 27kDa band in all human, mouse and rat tissue and cell line lysates tested after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 210kDa according to NP_004548.3. The 27kDa band was successfully blocked by incubation with the immunizing peptide. | |
Application Type Pep-ELISA, IF |
Goat Anti-NOTCH4 (aa1261-1273) Antibody
$423.00
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