Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names quinone reductase 1|phylloquinone reductase|menadione reductase|dioxin-inducible 1|diaphorase (NADH/NADPH) (cytochrome b-5 reductase)|azoreductase|NAD(P)H:quinone oxireductase|NAD(P)H menadione oxidoreductase 1, dioxin-inducible|NAD(P)H:Quinone acceptor oxidoreductase type 1|NAD(P)H menadione oxidoreductase 1, dioxin-inducible|DT-diaphorase|NAD(P)H:menadione oxidoreductase 1, dioxin-inducible 1|diaphorase (NADH/NADPH) (cytochrome b-5 reductase)|diaphorase (NADH/NADPH)|diaphorase-4|NMORI|NMOR1|DIA4|DHQU|QR1|DTD|NAD(P)H dehydrogenase, quinone 1|NQO1 | |
Usage Summary Immunofluorescence: Expression of the protein seen in the cytoplasm and nucleus of HepG2 cells. Recommended concentration: 10µg/ml. | |
Accession ID NP_000894.1; NP_001020604.1; NP_001020605.1 | |
Blocking Peptide EBP05370 | |
Immunogen Peptide with sequence C-SIPTDNQIKARK, from the C Terminus of the protein sequence according to NP_000894.1; NP_001020604.1; NP_001020605.1. | |
Product Comments This products is expected to recognize all three reported isoforms (NP_000894.1, NP_001020604.1 and NP_001020605.1). | |
Peptide Sequence C-SIPTDNQIKARK | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Rat, Dog, Pig | |
Reactive Species Human, Rat | |
Human Gene ID 1728 | |
Mouse Gene ID 18104 | |
Product Grade ![]() | |
IHC Results In paraffin embedded Human Kidney shows specific staining of the glomeruli. Recommended concentration: 2-4µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:64000. | |
Western Blot Approx. 30kDa band observed in lysates of cell lines U251 and HepG2 and in Human Kidney, Lung and Duodenum lysates, and approx. 30-32kDa band in Rat Kidney, Lung and Duodenum lysates (calculated MW of 30.9kDa according to Human NP_000894.1 and Rat NP_058696.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, WB, IF, IHC |
Goat Anti-NQO1 Antibody
$464.00
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