Goat Anti-NQO1 Antibody

$464.00

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SKU: EB05370
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
quinone reductase 1|phylloquinone reductase|menadione reductase|dioxin-inducible 1|diaphorase (NADH/NADPH) (cytochrome b-5 reductase)|azoreductase|NAD(P)H:quinone oxireductase|NAD(P)H menadione oxidoreductase 1, dioxin-inducible|NAD(P)H:Quinone acceptor oxidoreductase type 1|NAD(P)H menadione oxidoreductase 1, dioxin-inducible|DT-diaphorase|NAD(P)H:menadione oxidoreductase 1, dioxin-inducible 1|diaphorase (NADH/NADPH) (cytochrome b-5 reductase)|diaphorase (NADH/NADPH)|diaphorase-4|NMORI|NMOR1|DIA4|DHQU|QR1|DTD|NAD(P)H dehydrogenase, quinone 1|NQO1
Usage Summary
Immunofluorescence: Expression of the protein seen in the cytoplasm and nucleus of HepG2 cells. Recommended concentration: 10µg/ml.
Accession ID
NP_000894.1; NP_001020604.1; NP_001020605.1
Blocking Peptide
EBP05370
Immunogen
Peptide with sequence C-SIPTDNQIKARK, from the C Terminus of the protein sequence according to NP_000894.1; NP_001020604.1; NP_001020605.1.
Product Comments
This products is expected to recognize all three reported isoforms (NP_000894.1, NP_001020604.1 and NP_001020605.1).
Peptide Sequence
C-SIPTDNQIKARK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Rat, Dog, Pig
Reactive Species
Human, Rat
Human Gene ID
1728
Mouse Gene ID
18104
Product Grade
IHC Results
In paraffin embedded Human Kidney shows specific staining of the glomeruli. Recommended concentration: 2-4µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Approx. 30kDa band observed in lysates of cell lines U251 and HepG2 and in Human Kidney, Lung and Duodenum lysates, and approx. 30-32kDa band in Rat Kidney, Lung and Duodenum lysates (calculated MW of 30.9kDa according to Human NP_000894.1 and Rat NP_058696.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, IHC