Goat Anti-NRF2 (aa445-458) Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

NFE2L2| OTTHUMP00000207980| OTTHUMP00000205251| nuclear factor, erythroid derived 2, like 2| nuclear factor erythroid 2-related factor 2| nuclear factor (erythroid-derived 2)-like 2| NRF2| NF-E2-related factor 2| NFE2-related factor 2| HEBP1| nuclear factor erythroid 2-related factor 2| OTTHUMP00000207980| OTTHUMP00000205251| NFE2-related factor 2| NF-E2-related factor 2| HEBP1| NRF2| nuclear factor (erythroid-derived 2)-like 2|NFE2L2

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS and HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

NP_006155.2; NP_001138884.1; NP_001138885.1; NP_001300831.1;

EBP11042

Peptide with sequence C-KHSSRLEAHLTRDE, from the internal region of the protein sequence according to NP_006155.2; NP_001138884.1; NP_001138885.1; NP_001300831.1;.

This antibody is expected to recognize all reported isoforms (

C-KHSSRLEAHLTRDE

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog, Pig, Cow

Human

4780

18024

83619

Antibody detection limit dilution 1:64000.

Approx 70kDa band observed in Human Lung lysates and approx 75kDa in preliminary testing of Mouse and Rat Liver and KNRK, MDCK, and nuclear NIH3T3 cell lysate (calculated MW of 67.8kDa according to NP_006155.2). This molecular weight rate is routinely observed by other sources. Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.

Pep-ELISA, WB, IF, FC