Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names PEP1|Nsg1|neuron-specific protein family member 1|neuron specific gene family member 1|Neep21|Bsmrb|brain specific mRNA b|brain neuron cytoplasmic protein 1/2 | |
Usage Summary Immunocytochemistry: Primary DIV9 cells from Hippocampus E18 Rat embryos, stained for neuronal marker MAP2 in blue and for glial marker GFAP in green showed exclusive labelling of EB11637 (red) in the neurons only. Recommended concentration: 1-5µg/ml. | |
Accession ID NP_077042.2 | |
Blocking Peptide EBP11637 | |
Immunogen Peptide with sequence EKGTKQPLLEDGFD-C, from the N Terminus of the protein sequence according to NP_077042.2. | |
Peptide Sequence EKGTKQPLLEDGFD-C | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Cow | |
Reactive Species Mouse, Rat | |
Human Gene ID 27065 | |
Mouse Gene ID 18196 | |
Rat Gene ID 25247 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:128000. | |
Western Blot Approx 22kDa band observed in Mouse fetal Brain lysates (calculated MW of 20.9kDa according to NP_035072.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour. | |
Application Type Pep-ELISA, WB, ICC |
Goat Anti-Nsg1 / Neep21 Antibody
$423.00
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Selected References [{"pmid": 30680309, "intro": "This antibody has been successfully used in ICC on Human:", "title": "Neuron-Specific Gene 2 (NSG2) encodes an AMPA receptor interacting protein that modulates excitatory neurotransmission", "author": "Praveen Chander, Matthew J. Kennedy, Bettina Winckler and Jason P. Weick", "journal": "eNeuro 4 January 2019, ENEURO.0292-18.2018; DOI:https://doi.org/10.1523/ENEURO.0292-18.2018"}, {"pmid": 28874679, "intro": "This antibody has been successfully used in ICC on Rat:", "title": "The endosomal neuronal proteins Nsg1/NEEP21 and Nsg2/P19 are itinerant, not resident proteins of dendritic endosomes.", "author": "Chan Choo Yap, Laura Digilio, Lloyd McMahon & Bettina Winckler", "journal": "Scientific Reports, 7: 10481, DOI: 10.1038/s41598-017-07667-x (2017)"}, {"pmid": 26465886, "intro": "This antibody has been successfully used in ICC on Rat:", "title": "Neurons in Rat Cultures: Preponderance of Single- and Double-Positive Cells, and Cell Type-Specific Expression of Neuron-Specific Gene Family Members, Nsg-1\r\n(NEEP21) and Nsg-2 (P19).", "author": "Digilio L, Yap CC, Winckler B", "journal": "PLoS One. 2015 Oct 14;10(10):e0140010."}] |