Goat Anti-P27KIP1 / CDKN1B (C Terminus) Antibody

$423.00

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SKU: EB05061
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
CDKN1B|P27KIP1|cyclin-dependent kinase inhibitor 1B (p27, Kip1)|KIP1|CDKN4|cyclin-dependent kinase inhibitor 1B|p27(KIP1)|KIP1|p27|MEN1B|MEN4|P27KIP1
Usage Summary
Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from anonymous customer).
Accession ID
NP_004055.1
Blocking Peptide
EBP05061
Immunogen
Peptide with sequence C-EQTPKKPGLRRRQT, from the C Terminus of the protein sequence according to NP_004055.1.
Peptide Sequence
C-EQTPKKPGLRRRQT
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Cow, Dog
Reactive Species
Human
Human Gene ID
1027
Mouse Gene ID
12576
Rat Gene ID
83571
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 26-27kDa band observed in Human Breast and Prostate lysates (calculated MW of 22.1kDa according to NP_004055.1). The observed molecular weight corresponds to earlier findings in literature (Xia et al, Proc Natl Acad Sci U S A. 2005 Sep 27;102(39):14028-33; PMID: 16169901). Recommended concentration: 0.5-1.5µg/ml.
Application Type
Pep-ELISA, WB, IP