Goat Anti-P2RY1 (aa247-257) Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

P2RY1|purinergic receptor P2Y, G-protein coupled, 1|P2Y1|ATP recepto|P2 purinoceptor subtype Y1|P2Y purinoceptor 1|platelet ADP receptor|purinergic receptor P2Y1

NP_002554.1

EBP08201

Peptide with sequence C-KDLDNSPLRRK, from the internal region of the protein sequence according to NP_002554.1.

C-KDLDNSPLRRK

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog

5028

Antibody detection limit dilution 1:128000.

Preliminary experiments gave bands at approx 55kDa and 38kDa in lysates of cell lines HEK293 and A549 after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 42.1kDa according to NP_002554.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the observed sizes). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA