Goat Anti-parotid secretory protein (mouse aa146-160) Antibody

$423.00

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SKU: EB10621
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
Bpifa2e| OTTMUSP00000036473| OTTMUSP00000018314| parotid secretory protein|Psp
Usage Summary
Immunocytochemistry: A subset of murine submandibular gland cells (E18) show specific staining in secretory granules. Recommended concentration 4ug/ml. Data provided by Everest Grant winner Melinda Larsen, State University of New York, Albany, NY.
Accession ID
NP_032979.1
Blocking Peptide
EBP10621
Immunogen
Peptide with sequence C-KTNAQTGLPEVTIGK, from the internal region of the protein sequence according to NP_032979.1.
Peptide Sequence
C-KTNAQTGLPEVTIGK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Mouse
Reactive Species
Mouse
Mouse Gene ID
19194
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 25kDa band observed in Mouse mandibular glands from E17 onwards (calculated MW of 24.8kDa according to NP_032979.1). Recommended concentration: 1-3µg/ml. Data provided by Everest Grant winner Melinda Larsen, State University of New York, Albany, NY. This antibody has been successfully used in WB on Mouse, PMID: 32390232.
Application Type
Pep-ELISA, WB, ICC
Selected References
[{"pmid": 30310071, "intro": "This antibody has been successfully used in ICC/ IF on Mouse:", "title": "Generation of orthotopically functional salivary gland from embryonic stem cells.", "author": "Tanaka, J., Ogawa, M., et al.", "journal": "Nat Commun. 2018 Oct 11;9(1):4216."}, {"pmid": 26185754, "intro": "This antibody has been successfully used in IF on Mouse:", "title": "Regenerating Salivary Glands in the Microenvironment of Induced Pluripotent Stem Cells.", "author": "Ono H, Obana A, Usami Y, Sakai M, Nohara K, Egusa H, Sakai T.", "journal": "Biomed Res Int. 2015;2015:293570. doi: 10.1155/2015/293570. Epub 2015 Jun 22."}, {"pmid": 32390232, "intro": "This antibody has been successfully used in Western blot on Mouse:", "title": "The parotid secretory protein BPIFA2 is a salivary surfactant that affects lipopolysaccharide action", "author": "Seshagiri Rao Nandula, Ian Huxford, Thomas T. Wheeler, Conrado Aparicio and Sven?Ulrik Gorr", "journal": "Exp Physiol. 2020 May 11. doi: 10.1113/EP088567"}]