Goat Anti-PARP4 / VPARP Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

vault protein, 193-kDa|poly(ADP-ribosyl)transferase-like 1|poly(ADP-ribose) synthetase|PARP-related|OTTHUMP00000042288|H5 proline-rich I-alpha-I-related|ADP-ribosyltransferase (NAD poly ADP-ribose polymerase)-like 1|p193|VPARP|VAULT3|PH5P|PARPL|ADPRTL1|HGNC:271|poly (ADP-ribose) polymerase family, member 4|PARP4

NP_006428.1

EBP07100

Peptide with sequence DTHELKQKRTDC, from the internal region of the protein sequence according to NP_006428.1.

DTHELKQKRTDC

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog

143

Antibody detection limit dilution 1:16000.

Preliminary experiments gave an approx 80-85kDa band in Human Tonsil and HeLa lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 193kDa according to NP_006428.1. The 80-85kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA