Goat Anti-PCBP1 (aa223-234) Antibody

$423.00

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SKU: EB10497
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
alpha-CP1|heterogeneous nuclear ribonucleoprotein E1|heterogenous nuclear ribonucleoprotein E1|heterogenous nuclear ribonucleoprotein X|hnRNP-E1|hnRNP-X|HNRPE1|HNRPX|nucleic acid binding protein sub 2.3|poly(rC) binding protein 1|PCBP1
Usage Summary
Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Accession ID
NP_006187.2
Blocking Peptide
EBP10497
Immunogen
Peptide with sequence C-SIQGQHTISPLD, from the internal region of the protein sequence according to NP_006187.2.
Peptide Sequence
C-SIQGQHTISPLD
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog
Reactive Species
Human, Rat
Human Gene ID
5093
Mouse Gene ID
23983
Rat Gene ID
500242
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:8000.
Western Blot
Approx 38kDa band observed in Human Peripheral Blood Nucleocytes lysates (calculated MW of 37.5kDa according to NP_006187.2). The 38kDa was also observed in Rat Spleen while a 37kDa band was observed in Rat Thymus. Recommended concentration: 0.1-0.3µg/ml.
Application Type
Pep-ELISA, WB
Selected References
[{"pmid": 30377371, "intro": "This antibody has been successfully used in the following paper:", "title": "A high-throughput pipeline for validation of antibodies", "author": "Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen", "journal": "Nat Methods. 2018 Nov;15(11):909-912"}]