Goat Anti-PCDH11Y / PCDH11X Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

protocadherin-S|protocadherin X|protocadherin 11X|PCDHX|PCDH11|PCDH-X|RP13-138P15.1|protocadherin Y|protocadherin 22|protocadherin 11 X-linked|OTTHUMP00000033077|PCDHY|PCDH22|PCDH11X|PCDH-PC|protocadherin 11 Y-linked|PCDH11Y

NP_116753.1; NP_116754.1; NP_116755.1; NP_055337.1; NP_055337.1; NP_116750.1; NP_116751.1

EBP09719

Peptide with sequence C-KYNWVTTPTTFK, from the internal region of the protein sequence according to NP_116753.1; NP_116754.1; NP_116755.1; NP_055337.1; NP_055337.1; NP_116750.1; NP_116751.1.

This antibody is expected to recognize all reported Y-linked isoforms (NP_116753.1; NP_116754.1; NP_116755.1) and all reported X-linked isoforms (NP_055337.1; NP_055337.1; NP_116750.1; NP_116751.1).

C-KYNWVTTPTTFK

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human

Human

83259, 27328

Paraffin embedded Human Brain (Cortex). Recommended concentration: 6µg/ml.

Antibody detection limit dilution 1:8000.

Preliminary experiments gave an approx 75kDa band in Human Brain (Amygdala, Hippocampus, Substantia nigra) lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 115kDa according to NP_116753.1 and of 113kDa according to NP_055337.1, the smallest reported isoforms. The 75kDa band was successfully blocked by incubation with the immunizing peptide.

Pep-ELISA, IHC