Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
| |
Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names phosphopyruvate carboxylase|phosphoenolpyruvate carboxylase|mitochondrial phosphoenolpyruvate carboxykinase 2|PEP carboxykinase|PEPCK2|PEPCK|HGNC:8725|phosphoenolpyruvate carboxykinase 2 (mitochondrial)|PEPCK-M|PCK2 | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_004554.3; NP_001278485.1 | |
Blocking Peptide EBP06944 | |
Immunogen Peptide with sequence KPWKPGDKEPCAH, from the internal region of the protein sequence according to NP_004554.3; NP_001278485.1. | |
Product Comments No cross-reactivity with PCK1. | |
Peptide Sequence KPWKPGDKEPCAH | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog, Pig, Cow | |
Reactive Species Human, Mouse | |
Human Gene ID 5106 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:4000. | |
Western Blot Approx 70kDa band observed in lysates of cell lines A431, HEK293 HepG2 and NIH3T3 (calculated MW of 70.7kDa according to Human NP_004554.3 and 70.5kDa according to Mouse NP_083270.2). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, WB, IF, FC |
Goat Anti-PCK2 / PEPCK-M Antibody
$464.00
| SKU | Unit Size | Price |
|---|---|---|
Select a unit size:
Selected References [{"pmid": 23466304, "intro": "This antibody (previous batch) has been successfully used in WB and IF on Mouse:", "title": "PEPCK-M expression in mouse liver potentiates, not replaces, PEPCK-C mediated gluconeogenesis.", "author": "Méndez-Lucas A, Duarte JA, Sunny NE, Satapati S, He T, Fu X, Bermúdez J, Burgess SC, Perales JC.", "journal": "J Hepatol. 2013 Mar 4. doi:pii: S0168-8278(13)00142-6."}, {"pmid": 24973213, "intro": "This antibody (previous batch) has been successfully used in WB and IF on Human:", "title": "Mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M) is a pro-survival, endoplasmicreticulum (ER) stress response gene involved in tumor cell adaptation to nutrient availability.", "author": "Méndez-Lucas A, Hyrossová P, Novellasdemunt L, Viñals F, Perales JC.", "journal": "J Biol Chem. 2014 Aug 8;289(32):22090-102"}] |