Goat Anti-PDE5A Antibody

$464.00

Category: Primary Antibodies
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SKU: EB06645
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
cGMP-binding cGMP-specific 3\1, 5\1-cyclic nucleotide phosphodiesterase|phosphodiesterase 5A, cGMP-specific|CGB-PDE|PDE5A1|PDE5|CN5A|PDE5A
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_001074.2; NP_246273.2; NP_236914.2; (NP_237223)
Blocking Peptide
EBP06645
Immunogen
Peptide with sequence C-LINGESGQAKRN, from the C Terminus of the protein sequence according to NP_001074.2; NP_246273.2; NP_236914.2; (NP_237223).
Product Comments
This antibody is expected to recognise reported isoforms NP_001074.2, NP_236914.2 and NP_246273.2.
Peptide Sequence
C-LINGESGQAKRN
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Rat, Dog, Cow
Reactive Species
Human
Human Gene ID
8654
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
In transfected HEK293 transiently expressing PDE5A isoform 2 a band of approx. 95kDa is observed. This band is not observed in the non-transfected HEK293 (calculated MW of 94.8kDa according to isoform 2, NP_236914.2). Recommended concentration: 0.3-1µg/ml.
Application Type
Pep-ELISA, WB-Trf, IF, FC

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