Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names nucleoporin 85|Nup75|FLJ12549|nucleoporin 85kDa|nucleoporin Nup75|frount|PCNT|PCN|PCNT1|pericentrin 1|NUP85 | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm) of MCF7 and U2OS cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_079120.1; NP_001290205.1; NP_001317401.1 | |
Blocking Peptide EBP06201 | |
Immunogen Peptide with sequence C-ARAIIREGSLEGS, from the C Terminus of the protein sequence according to NP_079120.1; NP_001290205.1; NP_001317401.1. | |
Product Comments This antibody is expected to recognise al reported isoforms. | |
Peptide Sequence C-ARAIIREGSLEGS | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat | |
Reactive Species Human, Mouse | |
Human Gene ID 79902 | |
Mouse Gene ID 18541 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:32000. | |
Western Blot Approx. 75kDa band observed in lysates of cell line HeLa and approx. 70kDa in NIH3T3 cell lysates and in nuclear lysates of cell lines HeLa and HEK293 (calculated MW of 75.0kDa according to NP_079120.1). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature. Positive Control: A batch specific positive control lysate is available for this product. Please contact [email protected] for availability. | |
Application Type Pep-ELISA, WB, IF, FC |
Goat Anti-Pericentrin 1 / NUP85 Antibody
$464.00
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Selected References [{"pmid": 19841162, "intro": "This antibody has been successfully used in WB and IP on Human:", "title": "FROUNT is a common regulator of CCR2 and CCR5 signaling to control directional migration.", "author": "Toda E, Terashima Y, Sato T, Hirose K, Kanegasaki S, Matsushima K.", "journal": " J Immunol. 2009 Nov 15;183(10):6387-94."}, {"pmid": 2, "intro": "This antibody has been successfully used in IHC on Rat:", "title": "Disulfiram Ophthalmic Solution Inhibited Macrophage Infiltration by Suppressing Macrophage Pseudopodia Formation in a Rat Corneal Alkali Burn Model", "author": "Toyo Ikebukuro et al.", "journal": "Int. J. Mol. Sci. 2023, 24(1), 735; https://doi.org/10.3390/ijms24010735"}, {"pmid": 39384840, "intro": "This antibody has been successfully used in ICC on Mouse:", "title": "Antifibrotic effect of disulfiram on bleomycin-induced lung fibrosis in mice and its impact on macrophage infiltration", "author": "Yugo Okabe, Etsuko Toda, Hirokazu Urushiyama, Yuya Terashima, Shinobu Kunugi, Yusuke Kajimoto, Mika Terasaki, Kouji Matsushima, Akira Saito, Yasuhiro Yamauchi, Takahide Nagase, Akira Shimizu, Yasuhiro Terasaki", "journal": "Sci Rep. 2024 Oct 10;14(1):23653."}] |