Goat Anti-Praja-1 / RNF70 (aa285-298) Antibody

$255.00

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SKU: EB11464
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
PJA1| RNF70| RING finger protein 70| PRAJA1| praja ring finger 1| praja 1| OTTHUMP00000158771| OTTHUMP00000023458| OTTHUMP00000023457| E3 ubiquitin-protein ligase Praja-1
Accession ID
NP_660095.1; NP_001027568.1; NP_071763.2
Blocking Peptide
EBP11464
Immunogen
Peptide with sequence C-HSTSRWRDTANDNE, from the internal region of the protein sequence according to NP_660095.1; NP_001027568.1; NP_071763.2.
Product Comments
This antibody is expected to recognize all reported isoforms (NP_660095.1; NP_001027568.1; NP_071763.2).
Peptide Sequence
C-HSTSRWRDTANDNE
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse
Human Gene ID
64219
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:4000.
Western Blot
Preliminary experiments gave an approx 30kDa band in Human Brain and Mouse Brain lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 50.4kDa according to NP_071763.2. The 30kDa band was successfully blocked by incubation with the immunizing peptide.
Application Type
Pep-ELISA