Goat Anti-PRKD1 (aa233-246) Antibody

$464.00

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SKU: EB12885
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
PRKCM;|PKD|PKCM|PKC-MU|PRKD1
Usage Summary
Immunoprecipitation: PKD1 was immunoprecipitated from Hek293T cells (as above). Immunoprecipitates were analyzed by Western Blot probed with the same antibody.
Accession ID
NP_002733.2
Blocking Peptide
EBP12885
Immunogen
Peptide with sequence C-QKSPSESFIGREKR, from the internal region of the protein sequence according to NP_002733.2.
Peptide Sequence
C-QKSPSESFIGREKR
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Pig
Reactive Species
Human
Human Gene ID
5587
Mouse Gene ID
18760
Rat Gene ID
85421
Product Grade
IHC Results
This antibody has been confirmed in human pancreatic adenocarcinoma (PDA) according to Döppler et al, Sci Rep. 2016 Sep 21;6:33758 PMID 27649783.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
In transfected HEK293 transiently expressing Human PKD1 (DYKDDDDK tagged), a band of approx. 100kDa is observed. The same band is observed using anti- DYKDDDDK antibody and no bands are observed in cells transiently expressing Human PKD2 or PKD3. Recommended concentration 0.1-0.3ug/ml
Application Type
Pep-ELISA, WB-Trf, IP, IHC
Selected References
[{"pmid": 27649783, "intro": "This antibody has been successfully used in the following paper:", "title": "The PRKD1 promoter is a target of the Kras-NF-?B pathway in pancreatic cancer.", "author": "Döppler H, Panayiotou R, Reid EM, Maimo W, Bastea L, Storz P.", "journal": "Sci Rep.\r\n2016 Sep 21;6:33758."}]