Goat Anti-PTCH (Internal) Antibody

$423.00

Category: Primary Antibodies
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SKU: EB07312
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
patched (Drosophila) homolog|patched|PTCH protein -10|PTCH protein +4\1|PTCH protein +12b|PTCH1|PTC1|PTC|NBCCS|HPE7|FLJ42602|BCNS|RP11-435O5.3|patched homolog (Drosophila)|PTCH
Usage Summary
Immunofluorescence :Expression of the protein seen in the Golgi/membrane of NIH3T3 cells. Recommended concentration: 5µg/ml.
Accession ID
NP_000255.2; NP_001077072.1; NP_001077071.1; NP_001077075.1
Blocking Peptide
EBP07312
Immunogen
Peptide with sequence C-HPESRHHPPSNPRQQ, from the internal region of the protein sequence according to NP_000255.2; NP_001077072.1; NP_001077071.1; NP_001077075.1.
Product Comments
This antibody is expected to recognise all four reported isoforms (NP_000255.2; NP_001077072.1; NP_001077071.1; NP_001077075.1). Reported variants represent identical protein (NP_001077075.1; NP_001077076.1; NP_001077073.1; NP_001077074.1).
Peptide Sequence
C-HPESRHHPPSNPRQQ
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Dog, Pig, Cow
Reactive Species
Human, Mouse
Human Gene ID
5727
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 150kDa band observed in Human Brain lysates (calculated MW of 153.9kDa according to NP_001077071.1). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, IF, WB

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