Goat Anti-PU.1 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB08429
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
hematopoietic transcription factor PU.1|SPI-1 proto-oncogene|31 kDa transforming protein|SPI-A|SPI-1|SFPI1|hCG_25181|spleen focus forming virus (SFFV) proviral integration oncogene spi1|SPI1|PU.1
Accession ID
NP_001074016.1; NP_003111.2
Blocking Peptide
EBP08429
Immunogen
Peptide with sequence C-DLYQRQTHEYY, from the internal region of the protein sequence according to NP_001074016.1; NP_003111.2.
Product Comments
This antibody is expected to recognise both reported isoforms (NP_001074016.1; NP_003111.2).
Peptide Sequence
C-DLYQRQTHEYY
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Rat
Reactive Species
Human
Human Gene ID
6688
Rat Gene ID
366126
Product Grade
IHC Results
Paraffin embedded Human Spleen and Colon (lymphoid aggregate). Recommended concentration: 3.75µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 40kDa band observed in lysates of DAUDI and K562 (calculated MW of 31.2kDa according to NP_001074016.1 and of 31.1kDa according to NP_003111.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Harendza S et al, J Biol Chem. 2000 Jun 30;275(26):19552-9.; PMID: 10867017). Recommended concentration: 0.03-0.1µg/ml. Primary incubation was 1 hour.
Application Type
Pep-ELISA, WB, IHC

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