Goat Anti-PXR / NR1I2 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB09172
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
steroid and xenobiotic receptor|pregnane X receptor|orphan nuclear receptor|SXR|SAR|PRR|PARq|PAR2|PAR1|PAR|ONR1|BXR|nuclear receptor subfamily 1, group I, member 2|NR1I2|PXR
Accession ID
NP_003880.3; NP_071285.1; NP_148934.1
Blocking Peptide
EBP09172
Immunogen
Peptide with sequence EQFAITLKSYIECNR, from the internal region of the protein sequence according to NP_003880.3; NP_071285.1; NP_148934.1.
Product Comments
This antibody is expected to recognize all reported isoforms (NP_071285.1; NP_148934.1; NP_003880.3).
Peptide Sequence
EQFAITLKSYIECNR
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
8856
Product Grade
IHC Results
Paraffin embedded Human Small Intestine. Recommended concentration: 3.75µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:4000.
Western Blot
Approx. 48kDa band observed in lysates of cell line HepG2 (calculated MW of 49.8kDa according to NP_003880.3). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour.
Application Type
Pep-ELISA, WB, IHC

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