Goat Anti-RAD51D / RAD51L3 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

RAD51-like 3|Trad|DNA repair protein RAD51 homolog 4|recombination repair protein|RAD51D|HsTRAD|R51H3|TRAD|RAD51-like 3 (S. cerevisiae)|RAD51L3

NP_002869.3; NP_598332.1; NP_001136043.1

EBP05692

Peptide with sequence GVLRVGLCPGLTEE, from the N Terminus of the protein sequence according to NP_002869.3; NP_598332.1; NP_001136043.1.

This antibody is expected to recognise all three human isoforms of this protein.

GVLRVGLCPGLTEE

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human

5892

Antibody detection limit dilution 1:32000.

Preliminary experiments gave an approx 65kDa band in Human Brain and A431 lysates at 1ug/ml, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted sizes of between 38kDa and 6kDda according to NP_002869; NP_598330; NP_598331; NP_598332; NP_598333. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA