Goat Anti-RANBP1 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB06420
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
RAN binding protein 1|RANBP1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HEK293 and HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_002873.1; NP_001265568.1; NP_001265569.1; NP_001265570.1
Blocking Peptide
EBP06420
Immunogen
Peptide with sequence C-LSVKEETKEDAEEKQ, from the C Terminus of the protein sequence according to NP_002873.1; NP_001265568.1; NP_001265569.1; NP_001265570.1.
Product Comments
This antibody is expected to recognise reported isoforms 1, 2, 3 and 4.
Peptide Sequence
C-LSVKEETKEDAEEKQ
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
5902
Product Grade
IHC Results
In paraffin embedded Human Kidney shows pixulated cytoplasm staining in DCT. Recommended concentration: 3-5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx. 26-27kDa band observed in lysates of cell lines A431 HeLa and MCF7 and in Human Duodenum lysates, (calculated MW of 23.3kDa according to NP_002873.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.03-0.5µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC, IF, FC

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