Goat Anti-RASIP1 / RAIN Antibody

$423.00

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SKU: EB06422
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
Ras interacting protein 1|RASIP1|FLJ20401|RAIN|Ras-interacting protein 1
Accession ID
NP_060275.2
Blocking Peptide
EBP06422
Immunogen
Peptide with sequence CANYRHGPPVATSP, from the C Terminus of the protein sequence according to NP_060275.2.
Peptide Sequence
CANYRHGPPVATSP
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
54922
Product Grade
IHC Results
Paraffin embedded Human Placenta. Recommended concentration: 3.75µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:8000.
Western Blot
Experiments gave bands at approx 95kDa, 75kDa and 45kDa in A549 Lung Carcinoma lysate after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the additional bands we observe given the predicted size of 110kDa according to NP_060275. The detected bands were successfully blocked by incubation with the immunizing peptide.
Application Type
Pep-ELISA, IHC