Goat Anti-Restin / CLIP1 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB09512
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
restin (Reed-Steinberg cell-expressed intermediate filament-associated protein)|restin|cytoplasmic linker 1|Reed-Steinberg cell-expressed intermediate filament-associated protein|RSN|MGC131604|CYLN1|CLIP170|CLIP-170|CLIP|CAP-GLY domain containing linker protein 1|CLIP1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in A431 and A549 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_002947.1; NP_937883.1; NP_001234926.1; NP_001376220.1
Blocking Peptide
EBP09512
Immunogen
Peptide with sequence C-PHSTHHGSRGEERP, from the C Terminus of the protein sequence according to NP_002947.1; NP_937883.1; NP_001234926.1; NP_001376220.1.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_002947.1; NP_937883.1).
Peptide Sequence
C-PHSTHHGSRGEERP
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
6249
Mouse Gene ID
56430
Rat Gene ID
65201
Product Grade
IHC Results
Paraffin embedded Human Thyroid and Prostate. Recommended concentration: 2.5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:8000.
Western Blot
Approx. 150kDa band observed in lysates of cell lines HEK293, A549, HepG2 and HeLa (calculated MW of 161kDa according to NP_002947.1). Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC, IF, FC

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