Goat Anti-Ribosomal protein L22 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

heparin-binding protein HBp15|heparin-binding protein 15|Epstein-Barr-encoded RNA-associated protein|Epstein-Barr virus small RNA-associated protein|EBER-associated protein|60S ribosomal protein L22|HBP15/L22|HBP15|EAP|ribosomal protein L22|RPL22

NP_000974.1

EBP08104

Peptide with sequence C-SKESYELRYFQINQ, from the C Terminus of the protein sequence according to NP_000974.1.

C-SKESYELRYFQINQ

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat

Human

6146

19934

81768

In paraffin embedded Human Brain (Cortex) shows cytoplasm staining in neuronal bodies. Recommended concentration: 3-6µg/ml. Paraffin embedded Human Thyroid, Uterus and Testis. Recommended concentration: 3.75µg/ml.

Antibody detection limit dilution 1:16000.

Preliminary experiments gave bands at approx 17-18kDa and 25kDa in Human Liver lysates after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 14.8kDa according to NP_000974.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). This batch is not expected to cross-react with Mouse and Rat in Western blot.

Pep-ELISA, IHC