Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names UBC13/UEV-interacting ring finger protein|OTTHUMP00000039684|C3HC4-type zinc finger protein|FLJ12013|KIAA0646|ring finger protein (C3HC4 type) 8|ring finger protein 8|RNF8 | |
Usage Summary Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_003949.1; NP_898901.1 | |
Blocking Peptide EBP05843 | |
Immunogen Peptide with sequence GEPGFFVTGDRAG-C, from the N Terminus of the protein sequence according to NP_003949.1; NP_898901.1. | |
Product Comments This antibody is expected to recognize both reported isoforms (NP_003949.1 and NP_898901.1). | |
Peptide Sequence GEPGFFVTGDRAG-C | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human | |
Reactive Species Human | |
Human Gene ID 9025 | |
Product Grade ![]() | |
ELISA Detection Limit Antibody detection limit dilution 1:128000. | |
Western Blot Approx 55kDa band observed in Human Testis and Lung lysates and approx. 50kDa in nuclear lysates of cell lines HepG2 and Jurkat (predicted MW of 56kDa according to NP_003949.1 and 50.8kDa according to NP_898901.1 ). Recommended concentration 0.5-2µg/ml. Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, WB, FC |
Goat Anti-RNF8 Antibody
$423.00
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