Goat Anti-Robo3.1 (mouse aa1338-1351) Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

Rbig1|retinoblastoma inhibiting gene 1|retinoblastoma-inhibiting gene 1 protein|Rig1|Rig-1|Robo3|Robo3a|Robo3b|roundabout homolog 3|roundabout homolog 3 (Drosophila)

NP_001158239.1

EBP11775

Peptide with sequence C-PYGRPSFLSHGQGT, from the C-terminus of the protein sequence according to NP_001158239.1.

C-PYGRPSFLSHGQGT

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Mouse

19649

Antibody detection limit dilution 1:128000.

Preliminary experiments gave bands at approx 100kDa and 80kDa in Mouse fetal Brain lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 150kDa according to NP_001158239. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA