Goat Anti-RSPO3 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB13098
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Accession ID
NP_116173.2
Blocking Peptide
EBP13098
Immunogen
Peptide with sequence SGYYGTRYPDINKC, from the N Terminus of the protein sequence according to NP_116173.2.
Peptide Sequence
SGYYGTRYPDINKC
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat
Reactive Species
Human, Mouse, Rat
Human Gene ID
84870
Product Grade
IHC Results
Paraffin embedded Human Colon. Recommended concentration: 4-6µg/ml. Positive staining was also seen in paraffin embedded Human Kidney at 4ug/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 37kDa band observed in lysates of cell lines HeLa, U2OS, A549 and KNRK, and in Mouse Brain lysates (calculated MW of 30.9kDa according to Human NP_116173.2, and 31.4kDa according to Mouse NP_082627.3, and Rat NP_001094460.1). This molecular weight is observed by other sources, and was successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.1-0.3ug//ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC

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