Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names FLJ00318|FLJ00280|MGC102720|FLJ16733|FLJ16020|retinoid X receptor, alpha|NR2B1|RXRA | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and MCF7 cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_002948.1 | |
Blocking Peptide EBP06523 | |
Immunogen Peptide with sequence CQVNSSLTSPTGRGSM, from the internal region of the protein sequence according to NP_002948.1. | |
Product Comments This antibody is expected to recognise an epitope corresponding to aa 14-28 of human RXR alpha protein and does not cross-react with either RXR beta or gamma. | |
Peptide Sequence CQVNSSLTSPTGRGSM | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat | |
Reactive Species Human | |
Human Gene ID 6256 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:32000. | |
Western Blot Approx 55-60kDa band observed in nuclear lysates of cell lines HeLa and K562 (calculated MW of 50.8kDa according to NP_002948.1). This molecular weight is routinely observed by other sources and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, WB, IF, FC |
Goat Anti-RXR alpha Antibody
$464.00
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