Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
| |
Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names selenocysteine insertion sequence binding protein 2|OTTHUMP00000064932|OTTHUMP00000064931|OTTHUMP00000064930|OTTHUMP00000064929|DKFZp686C09169|SECIS binding protein 2|SECISBP2|RP11-89K14.1|SBP2 | |
Usage Summary Immunocytochemistry/Immunofluorescence: This antibody has been successfully used in ICC/IF for C33A cells as described in the following paper: Papp et al, Nucleic Acids Res. 2008 Dec;36(22):7192-206, PMID: 19004874. Immunoprecipitation: This antibody has also been successfully used for IP. | |
Accession ID NP_076982.3 | |
Blocking Peptide EBP07428 | |
Immunogen Peptide with sequence C-KERQERKQRLQEN, from the internal region of the protein sequence according to NP_076982.3. | |
Peptide Sequence C-KERQERKQRLQEN | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Dog, Pig, Cow | |
Reactive Species Human, Mouse | |
Human Gene ID 79048 | |
Product Grade  | |
IHC Results In paraffin embedded Human Testis shows granular cytoplasm staining in Leydig cells. Recommended concentration, 3µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:64000. | |
Western Blot Approx 120kDa band detected in HFF cell lysates. Data kindly provided by Laura Papp and Kum Kum Khanna. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Papp et al, Mol Cell Biol. 2006 Jul;26(13):4895-910; PMID: 16782878). Recommended concentration: 0.1-0.3µg/ml. This antibody has been successfully used in WB on Mouse: Raman AV et al. (2013) PMID: 24392277. This antibody has been successfully used in WB on Human: Papp LV et al. (2008) PMID: 19004874. | |
Application Type Pep-ELISA, WB, IP, IHC, ICC, IF |
Goat Anti-SBP2 Antibody
$464.00
| SKU | Unit Size | Price |
|---|---|---|
Select a unit size:
Selected References [{"pmid": 24392277, "intro": "This antibody has been successfully used in WB on Mouse:", "title": "Selenoprotein W expression and regulation in mouse brain and neurons.", "author": "Raman AV, Pitts MW, Seyedali A, Hashimoto AC, Bellinger FP, Berry MJ.", "journal": "Brain Behav. 2013 Sep;3(5):562-74."}, {"pmid": 19004874, "intro": "This antibody has been successfully used in WB and IF/ ICC on Human:", "title": "Functional characterization of alternatively spliced human SECISBP2 transcript variants.", "author": "Papp LV, Wang J, Kennedy D, Boucher D, Zhang Y, Gladyshev VN, Singh RN, Khanna KK.", "journal": "Nucleic Acids Res. 2008 Dec;36(22):7192-206."}] |