Goat Anti-SCRIB / Scribble Antibody

$423.00

Category: Primary Antibodies
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SKU: EB09387
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
scribble|Vartul|SCRIB1|SCRB1|CRIB1|scribbled homolog (Drosophila)|SCRIB
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cell junctions of MCF7 cells. Recommended concentration: 10µg/ml.
Accession ID
NP_874365.3; NP_056171.3
Blocking Peptide
EBP09387
Immunogen
Peptide with sequence C-PEGPGKEKELPGQ, from the internal region of the protein sequence according to NP_874365.3; NP_056171.3.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_874365.3; NP_056171.3).
Peptide Sequence
C-PEGPGKEKELPGQ
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
23513
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:1000.
Western Blot
Approx 200-250kDa band observed in lysates of cell lines HeLa, HepG2 and U2OS (calculated MW of 178kDa according to NP_874365.3). This molecular weight is routinely observed by other sources. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF

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