Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names PGL4|CWS2|SDH2|FLJ92337|iron-sulfur subunit|OTTHUMP00000044624|succinate dehydrogenase complex, subunit B, iron sulfur (Ip)|SDHIP|SDH1|SDH|IP|SDHB | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the plasm membrane/ cytoplasm of A431 cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_002991.2 | |
Blocking Peptide EBP06868 | |
Immunogen Peptide with sequence C-ATYKEKKASV, from the C Terminus of the protein sequence according to NP_002991.2. | |
Peptide Sequence C-ATYKEKKASV | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human | |
Reactive Species Human | |
Human Gene ID 6390 | |
Product Grade  | |
IHC Results Paraffin embedded Human Kidney. Recommended concentration: 6-8µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:8000. | |
Western Blot Approx 28-30kDa band observed in lysates of cell lines HEK293, Jurkat and HeLa (calculated MW of 31.6kDa according to NP_002991.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, WB, IHC, IF, FC |
Goat Anti-SDHB Antibody
$464.00
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