Goat Anti-Semaphorin 3E (internal) Antibody

$423.00

Category: Primary Antibodies
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SKU: EB07541
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
semaphorin 3E|sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3E|coll-5|SEMAH|M-sema H|M-SemaK|M-SEMAH|KIAA0331|sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E|SEMA3E
Accession ID
NP_036563.1; NP_001171600.1
Blocking Peptide
EBP07541
Immunogen
Peptide with sequence C-NGGRYGTTKDYPDD, from the internal region of the protein sequence according to NP_036563.1; NP_001171600.1.
Product Comments
This antibody is expected to recognise both reported isoforms (NP_036563.1; NP_001171600.1).
Peptide Sequence
C-NGGRYGTTKDYPDD
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Pig
Reactive Species
Human
Human Gene ID
9723
Mouse Gene ID
20349
Product Grade
IHC Results
In paraffin embedded Human Prostate shows membranous staining of secretory cells in the gland. Recommended concentration, 4-6µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Preliminary experiments gave an approx 35kDa band in Human Prostate lysates after 0.03µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 89.2kDa according to NP_036563.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide.
Application Type
Pep-ELISA, IHC

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