Goat Anti-SEPT4 (aa33-44) Antibody

$423.00

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SKU: EB10428
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
septin 4|SEP4|PNUTL2|peanut-like 2|MART|hucep-7|hCDCREL-2|H5|cerebral protein 7|cell division control-related protein 2|CE5B3|BRADEION|ARTS
Usage Summary

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Accession ID
NP_004565.1; NP_536340.1; NP_536341.1; NP_001185642.1
Blocking Peptide
EBP10428
Immunogen
Peptide with sequence C-ELSKFVKDFSGN, from the internal region of the protein sequence according to NP_004565.1; NP_536340.1; NP_536341.1; NP_001185642.1.
Product Comments
This antibody is expected to recognize all reported isoforms (NP_004565.1; NP_536340.1; NP_536341.1, NP_001185642.1). Amino acid numbering in name refers to NP_004565.1 sequence.
Peptide Sequence
C-ELSKFVKDFSGN
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse
Reactive Species
Human, Mouse
Human Gene ID
5414
Mouse Gene ID
18952
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx 55kDa band observed in nuclear lysates of cell lines Jurkat and in LNCaP cell lysates, 50-55kDa in Human Cerebellum lysates, and approx. 50kDa in Mouse Brain lysates (calculated MW of 53.0kDa according to Human NP_536341.1 and 49.4kDa according to Mouse NP_001271323.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, FC