Goat Anti-SHC1; SHC3 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

SHC (Src homology 2 domain-containing) transforming protein 1|SHCA|SHC|SHC (Src homology 2 domain containing) transforming protein 1|SHC1

Please note that the approx. 65-70kDa and 45kD bands correspond to two of the isoforms of SHC, as observed by Pelicci et al., [Cell 1992 Jul 10;70(1):93-104]. We also observed approx. 30-35kDa band. This lower MW band may be recognising an isoform with the accession number AAH33925. Moreover, splice prediction software (http://www.bioinformatics.ucla.edu/ASAP/) indicated the possibility of three isoforms for SHC1.

NP_003020; NP_058544

Peptide with sequence SELCLQQPVERKQ, from the C Terminus of the protein sequence according to NP_003020; NP_058544.

This product is expected to recognize the products of 2 different genes.

SELCLQQPVERKQ

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat

Human

6464, 53358

Western blot: Approx 65-70kDa, 45kDa and 30kDa bands seen in U937 lysate. Recommended for use at 1-3 µg/ml.